Platelet-Derived Mitochondria Attenuate 5-FU-Induced Injury to Bone-Associated Mesenchymal Stem Cells

Figure 2

5-FU impairs the hematopoietic regulatory ability of BA-MSCs. (a) Flow cytometric plots of BMCs cocultured with DMSO-treated BA-MSCs and 5-FU-treated BA-MSCs for 2 days. Flow cytometric plots are gated on lineage-negative BMCs and showing cell subsets of HPC (Lin^-Sca-1^-c-Kit⁺), HSC (Lin^-Sca-1⁺c-Kit⁺), LT-HSC (Lin^-Sca-1⁺c-Kit⁺CD34^lowFlk2^low), ST-HSC (Lin^-Sca-1⁺c-Kit⁺CD34⁺Flk2^low), MPP (Lin^-Sca-1⁺c-Kit⁺CD34⁺Flk2⁺), CMP (Lin^-Sca-1^-c-Kit⁺CD34⁺CD16/32^low), GMP (Lin^-Sca-1^-c-Kit⁺CD34⁺CD16/32⁺), and MEP (Lin^-Sca-1^-c-Kit⁺CD34^lowCD16/32^low) (). (b, c) Flow cytometry analysis of the percentage of HPC, HSC, LT-HSC, ST-HSC, MPP, CMP, GMP, and MEP in the BMCs cocultured with DMSO-treated BA-MSCs and 5-FU-treated BA-MSCs for 2 days (). (d) Gene expressions of Scf, Cxcl12, Thpo, Csfg, Csfm, Csfgm, and Il6 in DMSO-treated BA-MSCs and 5-FU-treated BA-MSCs were detected by qRT-PCR (). (e) Gene expressions of Scf, Cxcl12, Thpo, Csfg, Csfm, Csfgm, and Il6 in control mice and 5-FU-induced myelosuppression mice were detected by qRT-PCR (). All data are presented as . Statistics: unpaired Student’s -test (ns: not significant; , , and ).

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