Platelet-Derived Mitochondria Attenuate 5-FU-Induced Injury to Bone-Associated Mesenchymal Stem Cells

Figure 3

Analysis of mitochondrial dynamics in BA-MSCs after 5-FU chemotherapy. (a) TEM images depicting morphological changes of mitochondria (white rectangles) in control BA-MSCs and 5-FU-injured BA-MSCs. Scale bar, 2 μm, and 1 μm. (b) Gene expressions of Opa1, Mfn1, Mfn2, Drp1, Mff, Fis1, and Mid51 in DMSO-treated BA-MSCs and 5-FU-treated BA-MSCs were detected by qRT-PCR (). (c) Gene expression of Opa1, Mfn1, Mfn2, Drp1, Mff, Fis1, and Mid51 in control mice and 5-FU-induced myelosuppression mice were detected by qRT-PCR (). (d) Protein expression of DRP1 and OPA1 in DMSO-treated BA-MSCs and 5-FU-treated BA-MSCs were detected by western blotting. (e) Western blotting analysis of DRP1 and OPA1 in DMSO-treated BA-MSCs and 5-FU-treated BA-MSCs (). All data are presented as . Statistics: unpaired Student’s -test (ns: not significant; and ).

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