Platelet-Derived Mitochondria Attenuate 5-FU-Induced Injury to Bone-Associated Mesenchymal Stem Cells

Figure 5

Evaluation of the efficacy of platelet-derived mitochondria in the treatment of BA-MSCs injured by 5-FU. (a) Representative fluorescence images of BA-MSCs after 4 h and 8 h incubation with platelet-derived mitochondria. Mitochondria were previously labeled with MitoTracker Red CMXRos (red signal). The cytoplasm of BA-MSCs was labeled with CFSE (green signal), and the nucleus was labeled with DAPI (blue signal) before detection. (b) After incubation of 5-FU-injured BA-MSCs with platelet-derived mitochondria for 24 h, the apoptosis of BA-MSCs was analyzed by flow cytometry. (c) After incubation of 5-FU-injured BA-MSCs with platelet-derived mitochondria for 24 h, the cell cycle of BA-MSCs was analyzed by flow cytometry. (d, e) After incubation of 5-FU-injured BA-MSCs with platelet-derived mitochondria for 24 h, the MMP of BA-MSCs was analyzed by flow cytometry. (f) After incubation of 5-FU-injured BA-MSCs with platelet-derived mitochondria for 24 h, the ATP of BA-MSCs was analyzed. Control group: BA-MSCs were treated with DMSO for 48 h. 5-FU group: BA-MSCs were treated with 5-FU for 48 h. 5-FU + Mito group: BA-MSCs were treated with 5-FU for 48 h and incubated with platelet-derived mitochondria for 24 h. All data are presented as . Statistics: one-way ANOVA (ns: not significant; , , and ).

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