Platelet-Derived Mitochondria Attenuate 5-FU-Induced Injury to Bone-Associated Mesenchymal Stem Cells

Figure 6

Analysis of hematopoietic regulatory function of BA-MSCs treated with platelet-derived mitochondria after 5-FU chemotherapy. (a) Flow cytometry analysis of apoptosis of BMCs cocultured with BA-MSCs for 5 days. (b) Flow cytometry analysis of cell cycle of BMCs cocultured with BA-MSCs for 5 days. (c) Flow cytometric plots of BMCs cocultured with DMSO-treated BA-MSCs, 5-FU-treated BA-MSCs, and mitochondria-treated 5-FU-damaged BA-MSCs for 2 days. Flow cytometric plots are gated on lineage-negative BMCs and showing cell subsets of HPC, HSC, LT-HSC, ST-HSC, MPP, CMP, GMP, and MEP. (d, e) Flow cytometry analysis of percentage of HPC, HSC, LT-HSC, ST-HSC, MPP, CMP, GMP, and MEP cell subsets (). Control group: BA-MSCs were treated with DMSO for 48 h. 5-FU group: BA-MSCs were treated with 5-FU for 48 h. 5-FU + Mito group: BA-MSCs were treated with 5-FU for 48 h and incubated with platelet-derived mitochondria for 24 h. All data are presented as . Statistics: one-way ANOVA (ns: not significant; , , and ).

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