Molecular Mechanism of Long Noncoding RNA SNHG14 in Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells through the NEDD4L/FOXA2/PCP4 Axis

Figure 2

SNHG14 downregulation represses OD of BMSCs. BMSCs were treated with osteogenic induction (OI) and infected with SNHG14 shRNA lentivirus (sh-SNHG14), with NC shRNA (sh-NC) as the control. (a) Alkaline phosphatase (ALP) staining and ALP activity determination. (b) Alizarin red staining and determination of mineralized nodules. (c) expression levels of OCN and OPN were determined by Western blot analysis. (d–e) SNHG14 expression level was determined by RT-qPCR. Cell experiments were repeated 3 times. Data were represented as deviation. Multigroup comparisons in panels (a), (b), and (d) were analyzed using one-way ANOVA, and multigroup comparisons in panel (c) were analyzed by two-way ANOVA, followed by Tukey’s multiple comparison test; pairwise comparisons in panel (e) were analyzed using the -test. , .

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