Molecular Mechanism of Long Noncoding RNA SNHG14 in Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells through the NEDD4L/FOXA2/PCP4 Axis

Figure 3

SNHG14 binds to HuR to stabilize NEDD4L expression. (a, b) Subcellular localization of SNHG14 was analyzed by the subcellular fractionation assay and RNA FISH. (c) Binding probabilities of SNHG14 and HuR, HuR and NEDD4L were predicted on the RPISeq database. (d) Bindings of SNHG14 to HuR and HuR to NEDD4L were analyzed by RIP assay. (e, f) NEDD4L expression level was determined by RT-qPCR and Western blot analysis; BMSCs were infected with HuR shRNA lentivirus (sh-HuR), with sh-NC as the negative control, (g, h) Expression levels of HuR and NEDD4L were determined by RT-qPCR and Western blot analysis. (i) Half-life period of NEDD4L was determined by RT-qPCR. Cell experiments were repeated 3 times. Data were represented as deviation. Multigroup comparisons in panels (d)–(f) were analyzed using one-way ANOVA, and pairwise comparisons in panels (g)–(i) were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test. , .

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