EVL Promotes Osteo-/Odontogenic Differentiation of Dental Pulp Stem Cells via Activating JNK Signaling Pathway

Figure 3

Overexpression of EVL promoted the osteo-/odontogenic differentiation of hDPSCs. (a, b) Verification of the EVL overexpression efficiency in hDPSCs transfected with pM-EVL or pMV by western blot and qRT-PCR. (c) The expression of DSPP, DMP1, RUNX2, OCN, and OSX in hDPSCs transfected with pM-EVL and pMV by western blot on the 7th day. (d) The expression of DSPP, DMP1, ALP, RUNX2, OSX, OCN, and COL1A1 were analyzed in hDPSCs transfected with pM-EVL and pMV by qRT-PCR on the 3rd and 7th day. (e) ALP staining and quantitative intracellular ALP activity were determined in hDPSCs transfected with pM-EVL and pMV and cultured in GM or OM on the 3rd and 7th day. (f) Extracellular calcium deposition was visualized by alizarin red staining on the 21st day. Released dye in alizarin red staining was quantified by measuring absorbance at 562 nm. GAPDH was used as an internal control in qRT-PCR and western blot analyses. Data are shown as the (); , , .

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