EVL Promotes Osteo-/Odontogenic Differentiation of Dental Pulp Stem Cells via Activating JNK Signaling Pathway

Figure 5

EVL enhanced hDPSCs osteo-/odontogenic differentiation via activating JNK signaling. (a) Pretreatment with 20 or 40 μM SP600125 for 1 h, ALP staining and quantitative intracellular ALP activity were determined in hDPSCs transfected with pM-EVL on the 7th day. (b) Extracellular calcium deposition was visualized by alizarin red staining on the 21st day. Released dye in alizarin red staining was quantified by measuring absorbance at 562 nm. (c) Pretreatment with 20 or 50 μM SB203580 for 1 h, ALP and ARS staining were determined in hDPSCs transfected with pM-EVL. (d) Pretreatment with 20 or 40 μM SP600125 for 1 h, the expression of DSPP, DMP1, ALP, RUNX2, OSX, OCN, and COL1A1 is analyzed in hDPSCs transfected with pM-EVL by qRT-PCR on the 7th day. (e) The expression of DSPP, DMP1, RUNX2, OCN, and OSX in hDPSCs transfected with pM-EVL is analyzed by western blot on the 7th day. GAPDH was used as an internal control in qRT-PCR and western blot analyses. Data are shown as the (); , , .

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