Melatonin Promotes BMSCs Osteoblastic Differentiation and Relieves Inflammation by Suppressing the NF-κB Pathways

Figure 2

Melatonin promotes osteogenic differentiation of BMSCs. (a) Calcium deposits were visualized by alizarin red staining. Scale bar: 100 μm. (b) ALP staining was used to evaluate ALP activity. Scale bar: 100 μm. (c) RT-PCR was used to quantify the expression of osteogenic genes in BMSCs, such as ALP, Col 1, OCN, OPN, and RUNX2. (d, e) Western blotting was used to investigate the protein expressions of RUNX2 and OCN. MT-50: 50 μM melatonin; MT-100: 100 μM melatonin. The data are expressed as the from three independent experiments. versus the control (Con) group.

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