Melatonin Promotes BMSCs Osteoblastic Differentiation and Relieves Inflammation by Suppressing the NF-κB Pathways

Figure 4

Melatonin suppressed TNF-α-induced NF-κB signaling pathway activation in BMSCs. (a) Western blotting was used to investigate NF-κB pathway-related proteins such as p65 and p-p65. (b) Quantitative analysis of the protein levels of p-p65/p65. (c) Western blotting was used to investigate NF-κB pathway-related proteins such as IkBα and p-IkBα. (d) Quantitative analysis of the protein levels of p-IkBα/IkBα. Melatonin: 100 μM; TNF-α: 10 ng/mL. The data are expressed as the from three independent experiments. versus the control (Con) group.

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