Melatonin Promotes BMSCs Osteoblastic Differentiation and Relieves Inflammation by Suppressing the NF-κB Pathways

Figure 5

Melatonin reverses the inhibitory effect of TNF-α on osteogenic differentiation in BMSCs. (a) Calcium deposits were observed by alizarin red staining. Scale bar: 100 μm. (b) ALP staining was used to evaluate the ALP activity. Scale bar: 100 μm. (c-e) Western blotting was used to investigate the protein expression of RUNX2 and OCN. Melatonin: 100 μM; TNF-α: 10 ng/mL. The data are expressed as the from three independent experiments. versus the control (Con) group.

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