Melatonin Promotes BMSCs Osteoblastic Differentiation and Relieves Inflammation by Suppressing the NF-κB Pathways

Figure 6

Melatonin relieves the inhibitory effect of TNF-α on osteogenic and inflammatory gene expression. (a–e) RT-PCR was used to detect the expression of osteogenic genes such as ALP, Col 1, OCN, OPN, and RUNX2 in BMSCs. (f) RT-PCR was used to detect the proinflammatory IL-1β gene expression in BMSCs. (g) RT-PCR was used to detect the anti-inflammatory IL-10 gene expression in BMSCs. Melatonin: 100 μM; TNF-α: 10 ng/mL. Data are expressed as the from three independent experiments. versus the control (Con) group.

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