hUMSCs Restore Uterine Function by Inhibiting Endometrial Fibrosis via Regulation of the MMP-9/TIMP-1 Ratio in CDDP-Induced Injury Rats

Figure 4

Western blot analyses of uterine tissue fibrosis-related, MMP-9, and TIMP-1 proteins. (a) Uterine expressions of Collagen I, Collagen III, CTGF, Fibronectin, α-SMA, MMP-9, and TIMP-1 in each group. A representative blot from one of the three independent experiments performed is shown. GAPDH was used as the loading control. (b) Expressions of Collagen I, Collagen III, CTGF, Fibronectin, and α-SMA protein as quantitated using ImageJ software. (c) Levels of MMP-9, TIMP-1, and the MMP-9/TIMP-1 ratio in each group. Data are presented as the . . CDDP: cisplatin; Collagen I: Collagen Type I; Collagen III: Collagen Type III; CTGF: connective tissue growth factor; α-SMA: alpha smooth muscle actin; MMP-9: matrix metalloprotein 9; TIMP-1: tissue inhibitor of metalloproteinase-1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

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