hUMSCs Restore Uterine Function by Inhibiting Endometrial Fibrosis via Regulation of the MMP-9/TIMP-1 Ratio in CDDP-Induced Injury Rats

Figure 6

Fibrosis, MMP-9, and TIMP-1 expressions in hEnSCs. (a) Cell viability of hEnSCs as determined in response to varying concentrations of CDDP. (b) Expressions of Collagen I, Collagen III, CTGF, Fibronectin, α-SMA, MMP-9, and TIMP-1 in hEnSCs within each group. A representative blot from one of the three independent experiments performed is shown. GAPDH was used as the loading control. (c) Levels of Collagen I, Collagen III, CTGF, Fibronectin, and α-SMA within each group. (d–f) Levels of MMP-9, TIMP-1, and MMP-9/TIMP-1 ratio within each group. Data are presented as the . . CDDP: cisplatin; Collagen I: Collagen Type I; Collagen III: Collagen Type III; CTGF: connective tissue growth factor; α-SMA: alpha smooth muscle actin; MMP-9: matrix metalloprotein 9; TIMP-1: tissue inhibitor of metalloproteinase-1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

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