Human Platelet-Rich Plasma Regulates Canine Mesenchymal Stem Cell Migration through Aquaporins

Figure 4

hPRP effect on cMSC AQPs. cMSCs were treated with 10% hPRP-conditioned medium for 24 h. Cell lysates (20 μg protein/sample) were blotted with AQP1 (a) and AQP5 (b) antibodies. To ensure equal protein transfer, membranes were blotted with vinculin antibodies. Filters were revealed by enhanced chemiluminescence (ECL) and autoradiography. The autoradiographs shown are representative of three independent experiments. Densitometric analyses have been performed on autoradiographs. Bars show the ratio between AQPs and vinculin protein levels as a fold-over control (cells in 0.25% BSA). The data were analyzed using the Mann–Whitney test ().

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