Human Platelet-Rich Plasma Regulates Canine Mesenchymal Stem Cell Migration through Aquaporins

Figure 5

Effect of TEAC inhibitor. (a) cMSCs were treated with three different concentrations of TEAC (10 μM, 50 μM, and 100 μM) in DMEM F12 0.25% BSA medium. Cell viability was analyzed by the sulforodamine assay. Bars show the viability compared to control cells (those cultured in 0.25% BSA). The data obtained represent the of four independent experiments. (b) cMSCs were seeded in the upper chamber of a transwell culture system in the presence of 10% hPRP in the lower chamber with or without 100 μM TEAC. Cells that migrated across the filter were determined by crystal violet staining. Bars show the fold-over control (cells in the presence of 10% hPRP). Before crystal violet elution, cells were photographed (magnification 10x). The data represent the of three independent experiments ().

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