Transplantation of Umbilical Cord-Derived Mesenchymal Stem Cells Attenuates Surgical Wound-Induced Blood-Brain Barrier Dysfunction in Mice

Figure 1

UC-MSCs alleviated extravascular dextran level at different time points induced by surgical wound in mice. (a) The blood vessels were showed green at the first line, and dextran was showed red at the second line. The brain section of control group (the first column), the S-W (surgical wound group) after 6 hours (the second column), IL-6-AB after surgical wound (the third column), UC-MSCs at 1 hour after surgical wound (the fourth column), UC-MSCs at 6 hours (the fifth column), 12 hours (the sixth column), and 18 hours before surgical wound (the seventh column). The red spots of nonoverlap showed in the third line indicate the dextran outside the BMECs. in each group. Scale bar, 50um. (b) Fluorescent quantitation showed that different time points of UC-MSCs and IL-6-AB decreased extravascular level of 10 kDa dextran outside the BMECs compared with the S-W group (black bar) after surgical wound, and the most efficient level is the administration of UC-MSCs before surgical wound. in each group (for comparing with control, , , ).

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