METTL3 Promotes Osteo/Odontogenic Differentiation of Stem Cells by Inhibiting miR-196b-5p Maturation

Figure 1

miR-196b-5p suppressed the osteo/odontogenic differentiation potential of SCAPs in vitro. (a) The mRNA expression of miR-196b-5p was detected by real-time RT-PCR after mineralization induction at 3 and 7 days. (b) Real-time RT-PCR showed the knockdown efficiency of miR-196b-5p in SCAPs. (c) ALP activity assay demonstrating that miR-196b-5p knockdown enhanced ALP activity in SCAPs. (d) Alizarin red staining and (e) quantitative calcium analysis results demonstrating that miR-196b-5p knockdown enhanced mineralization in SCAPs (scale bar: 50 μm). (f) Western blot result demonstrating that the knockdown of miR-196b-5p upregulated the expression of DSPP and OCN in SCAPs. (g) Real-time RT-PCR showed the efficiency of miR-196b-5p overexpression in SCAPs. (h) ALP activity assay demonstrating that miR-196b-5p overexpression inhibited ALP activity in SCAPs. (i) Alizarin red staining and (j) quantitative calcium analysis results demonstrating that miR-196b-5p overexpression weakened mineralization in SCAPs (scale bar: 50 μm). (k) Western blot result demonstrating that the overexpression of miR-196b-5p downregulated the expression of DSPP and OCN in SCAPs. U6 or GAPDH was used for standardization. Student’s -test or one-way ANOVA was used to analyze the statistical significance. All error bars signify standard deviations (). and .

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