METTL3 Promotes Osteo/Odontogenic Differentiation of Stem Cells by Inhibiting miR-196b-5p Maturation

Figure 3

METTL3 promoted the osteo/odontogenic differentiation of SCAPs. (a–c) The protein and mRNA expressions of METTL3 were confirmed by western blot and real-time RT-PCR in miR-196b-5p mimic and in miR-196b-5p inhibitor. (d) The overexpression efficiency of METTL3 was confirmed by western blot. (e) ALP activity assay indicated that METTL3 overexpression enhanced ALP activity in SCAPs. (f) Alizarin red staining and (g) quantitative calcium analysis results demonstrated that METTL3 overexpression enhanced mineralization in SCAPs (scale bar: 50 μm). (h) Western blot result demonstrating that METTL3 overexpression upregulated the expression of DSPP and OCN in SCAPs. (i) The knockdown efficiency of METTL3 was confirmed by western blot. (j) ALP activity assay indicated that METTL3 knockdown inhibited ALP activity in SCAPs. (k) Alizarin red staining and (l) quantitative calcium analysis results demonstrating that METTL3 knockdown inhibited mineralization in SCAPs (scale bar: 50 μm). (m) Western blot result demonstrating that METTL3 knockdown downregulated the expression of DSPP and OCN in SCAPs. GAPDH was used for standardization. Student’s -test was used to analyze the statistical significance. All error bars signify standard deviations (). and .

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