METTL3 Promotes Osteo/Odontogenic Differentiation of Stem Cells by Inhibiting miR-196b-5p Maturation

<div>Cycloleucine upregulated miR-196b-5p levels and inhibited the osteo/odontogenic differentiation of SCAPs. (a) Effect of cycloleucine (10 mM) on the expression of miR-196b-5p. (b) ALP activity assay indicated that 10 mM CL repressed the knockdown of miR-196b-5p promoting ALP activity of SCAPs. (c) Alizarin red staining and (d) quantitative calcium analysis results demonstrating that 10 mM CL suppressed the enhanced mineralization by knockdown miR-196b-5p. One-way ANOVA was used to analyze the statistical significance. All error bars signify standard deviations (<span class="inline" id="M32"></span>). <span class="inline" id="M33"></span> and <span class="inline" id="M34"></span>.</div>

Stem Cells International

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Figure 5

Figure 5: METTL3 Promotes Osteo/Odontogenic Differentiation of Stem Cells by Inhibiting miR-196b-5p Maturation 