Spermidine Retarded the Senescence of Multipotent Mesenchymal Stromal Cells In Vitro and In Vivo through SIRT3-Mediated Antioxidation

Figure 1

Primary cell separation and cell viability test. (a) Structural and molecular formulas of small molecule compound spermidine. (b) Primary hUCMSCs harvested by the tissue block method and identified by our existing methods [35]. (c) Cell viability was tested with CCK-8, different concentrations of spermidine were added to the medium, and absorbance was measured using a microplate reader after 12 h. Cells were seeded in 96-well plates at a concentration of 10⁴ cells per well. All quantitative data were obtained from three independent experiments and presented as ; indicates a significant difference between the specified groups. N.S.: not significant;  μm.

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