Spermidine Retarded the Senescence of Multipotent Mesenchymal Stromal Cells In Vitro and In Vivo through SIRT3-Mediated Antioxidation

Figure 3

SPD intervention contributed to adipogenic and osteogenic differentiation in later-passage hUCMSCs. (a) Osteogenic and adipogenic differentiation was, respectively, observed 14 days and 21 days after treatment with the corresponding induced medium. Approximately 10⁵ cells per well were seeded in 6-well plates. Adipocytes were stained with Oil Red O, and osteoblasts were stained with Alizarin Red S. (b) The absorbance value was detected at 500 nm (Oil Red O) or 520 nm (Alizarin Red S) and then quantitatively analyzed between different groups. (c) Representative western blotting results indicated the protein expression levels of FABP4, PPARγ, ALP, or RUNX2 in various groups. (d) Protein expression levels detected by western blotting were quantified using ImageJ software and normalized to PDL6. All quantitative data were obtained from three independent experiments and presented as ; and imply a significant difference between the indicated groups,  μm.

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