Abnormal Decrease of Macrophage ALKBH5 Expression Causes Abnormal Polarization and Inhibits Osteoblast Differentiation

Figure 4

ALKBH5 promotes hMSC osteogenic differentiation. (a) ALKBH5 levels of cell transfected with or without sh-ALKBH5 were detected by Western blot (WB). (b) ALKBH5 mRNA levels of cell transfected with or without sh-ALKBH5 were detected by qPCR. (c) Dot-blot analysis of RNA m⁶A modification levels of cell transfected with or without sh-ALKBH5. MB, methyl blue. (d) hMSC osteogenic differentiation analyzed by Alizarin red–S. Scale bar, 50 μm. (e) Quantification of Alizarin red–S staining area in different groups. (f) Col1a1, OSX, and Runx2 mRNA levels of cell transfected with or without sh-ALKBH5 were detected by qPCR. Significance was defined as .

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