Mesenchymal Stem Cells Accelerate Recovery of Acetic Acid-Induced Chronic Gastric Ulcer by Regulating Ekt/Akt/TRIM29 Axis

Figure 6

The hUC-MSCs significantly increased expression of Erk/Akt/TRIM29 in vivo and in vitro: (a, b) volcano plots and heat map to determine all differentially expressed genes with a P-value of <0.05 between control group and MSC-CM treatment group in GES-1; (c) the TRIM29 mRNA expression in GES-1; (d, e) volcano plots and cluster analysis to show the differentially expressed proteins between the Model and MSC groups in mice; (f) the Trim29 protein expression between the Model and MSC group in mice; (g) verify the changes in related genes by qPCR in GES-1; (h) verify the expression of related proteins by western blot in GES-1; (i) changes in p-Erk1/2 and p-Akt levels in GES-1 cells treated with MSC-CM within 6 hr; (j, k) immunoblots of TRIM29, Erk1/2, p-Erk1/2, Akt, and p-Akt in gastric tissues. The density of western blot bands was quantified by ImageQuant TL software; (l, m) Immunohistochemical and quantitative results of TRIM29 in gastric tissues. The positive cells ratio was quantified by Aipathwell software.

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