Stem Cells International

Towards Creating the Perfect In Vitro Cell Model


Publishing date
01 Jan 2016
Status
Published
Submission deadline
14 Aug 2015

Lead Editor

1Genome Institute of Singapore, Singapore

2UMC Utrecht, Utrecht, Netherlands

3University of Skövde, Skövde, Sweden

4Sahlgrenska University Hospital, Molndal, Sweden


Towards Creating the Perfect In Vitro Cell Model

Description

Researchers regularly rely on in vitro models to study physiological and pathological processes related to human health and disease. These models range from relatively simple overexpression systems in, for example, HEK293 or COS cells, to more complex specialized cells/tissue/organs from animals or, in rare cases, humans (e.g., skin models). Translation of the results from bench to bedside is sometimes challenging; nevertheless, in vitro models are the foundation of contemporary research. Though a complete abolishment of animal models is highly unlikely in the near future, the importance of cell models will increase as initiatives like the 3Rs (refining, reducing, and replacing animals models) are gaining importance and the FDA is planning to adapt its guidelines for preclinical cardiotoxicity studies by incorporating a computational integration of various individual ion channel assays as well as electrophysiological tests on stem cell-derived cardiomyocytes. Needless to say, a continuous improvement of our in vitro models is therefore needed.

The discovery of (induced pluripotent) stem cells and their ability to differentiate into a large variety of different cell types has raised the hope for increasingly reliable in vitro models of human origin which includes the possibility to further explore their potential for personalized medicine. Extensive evaluation of these models has indeed shown great promise but has at the same time also identified several important limitations that currently hinder their implementation in routine assays. One of the most important problems still related to stem cell-derived models is an immature phenotype of the specialized cell, and this leaves consequently a too big gap to the in vivo situation. For this special issue, we invite investigators to contribute original research articles as well as review articles that will help the research community in approaching the goal of obtaining models for the lab that can mirror the in vivo situation.

Potential topics include, but are not limited to:

  • Advances in steering in Vitro cell differentiation: biomarkers for monitoring the differentiation process and for identifying/purifying intermediate and end-stage cell types, characterization of cell populations, and upscaling of harvest
  • New or improved methods to directly reprogram somatic cells into specialized cell types
  • Advanced model systems and culture platforms: semi-3D culture systems (coatings and scaffolds) as well as full 3D systems (organoids, tissue engineering) and the combination of different specialized cell types within one assay
  • Improvements in cell culture to simulate natural environments: hypoxia, extracellular matrix, culture at air-liquid interfaces, continuous flow of media, and so forth
  • The role of epigenetics and small noncoding RNAs during differentiation
  • Precise genomic engineering to generate disease models and cellular tools
  • Computer models: creation of in silico models or real-time interfaces between a living cell and a computer, such as a dynamic clamp

Articles

  • Special Issue
  • - Volume 2016
  • - Article ID 3459730
  • - Editorial

Towards Creating the Perfect In Vitro Cell Model

Malin K. B. Jonsson | Toon A. B. van Veen | ... | Bruno Becker
  • Special Issue
  • - Volume 2016
  • - Article ID 8648356
  • - Research Article

Highly Synchronized Expression of Lineage-Specific Genes during In Vitro Hepatic Differentiation of Human Pluripotent Stem Cell Lines

Nidal Ghosheh | Björn Olsson | ... | Jane Synnergren
  • Special Issue
  • - Volume 2016
  • - Article ID 5481493
  • - Research Article

Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes

Angela Maria Cozzolino | Valeria Noce | ... | Laura Amicone
  • Special Issue
  • - Volume 2016
  • - Article ID 8140168
  • - Review Article

The Rise of CRISPR/Cas for Genome Editing in Stem Cells

Bing Shui | Liz Hernandez Matias | ... | Ying Peng
  • Special Issue
  • - Volume 2016
  • - Article ID 6939438
  • - Research Article

PTF1a Activity in Enriched Posterior Foregut Endoderm, but Not Definitive Endoderm, Leads to Enhanced Pancreatic Differentiation in an In Vitro Mouse ESC-Based Model

Gopika G. Nair | Jon S. Odorico
  • Special Issue
  • - Volume 2016
  • - Article ID 2030478
  • - Research Article

Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering

Stefano Focaroli | Gabriella Teti | ... | Mirella Falconi
  • Special Issue
  • - Volume 2016
  • - Article ID 2475631
  • - Research Article

High Content Analysis of Human Pluripotent Stem Cell Derived Hepatocytes Reveals Drug Induced Steatosis and Phospholipidosis

Arvind Pradip | Daniella Steel | ... | Josefina Edsbagge
  • Special Issue
  • - Volume 2016
  • - Article ID 4148093
  • - Research Article

Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture

Olga Schmal | Jan Seifert | ... | Gerd Klein
  • Special Issue
  • - Volume 2016
  • - Article ID 4379425
  • - Review Article

Stem Cell Models to Investigate the Role of DNA Methylation Machinery in Development of Neuropsychiatric Disorders

K. Naga Mohan
  • Special Issue
  • - Volume 2016
  • - Article ID 6429853
  • - Research Article

p53-Dependent Senescence in Mesenchymal Stem Cells under Chronic Normoxia Is Potentiated by Low-Dose γ-Irradiation

Ines Höfig | Yashodhara Ingawale | ... | Michael Rosemann
Stem Cells International
 Journal metrics
Acceptance rate45%
Submission to final decision76 days
Acceptance to publication35 days
CiteScore3.800
Impact Factor3.902
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