Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene
Figure 18
(a) Amount of attached HRP protein on untreated (blue) and PIII-treated (red) polyethylene in dependence on pH of HRP solution before (empty signs) and after washing in SDS detergent during 1 hour at 70°C (full signs). The amount of attached protein does not depend on pH of buffer solution. (b) Amount of attached HRP protein on untreated (blue) and PIII-treated (red) polyethylene in dependence on salt NaCl concentration of HRP solution before (empty signs) and after washing in SDS detergent during 1 hour at 70°C (full signs). The amount of attached protein does not depend on salt concentration. (c) Amount of attached HRP protein on untreated (blue) and PIII-treated (red) polyethylene in dependence on concentration of HRP solution before (empty signs) and after washing in SDS detergent during 1 hour at 70°C (full signs). (d) Amount of attached HRP protein on polyethylene in dependence on PIII treatment time before (empty signs) and after washing in SDS detergent (full signs). The saturation of protein amount with PIII treatment time is observed for total protein amount and for amount of covalently attached protein. Low amount of free radicals is enough to achieve the saturation stage of protein attachment.
