Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene

Table 5

Amount of attached HRP protein (see (5)) on PIII-treated UHMWPE with various blocking agents of the surface. Detergent SDS was applied for analysis of covalently attached protein. The average error bar for the protein amount is 0.0010.

Blocking agent	Formula	As attached	After SDS washing
No blocking		0.0164	0.0113
Hexene	CH3(CH2)3CH=CH2	0.0162	0.0090
Styrene		0.0156	0.0098
Ethylenediamine		0.0122	0.0049
Polytetrahydrofuran (PTHF)		0.0102	0.0060
TEMPO		0.0119	0.0015
Benzyl mercaptan		0.0078	0.0018