Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene

Table 6

Amount of polyamino acids (see (5)) on PIII-treated polyethylene immediately after incubation (as attached) and after SDS washing (except for Poly-l-tryptophan, Poly-L-arginine, Poly-DL-alanine, and Poly-L-isoleucine as described in the text). The average error bar for the polyamino acid amount is 0.0005.

Aminoacid	Polyaminoacid	Side-chain groups	As; attached	After detergent washing
Glycine	Poly-L-glycine (in buffer)	–CH2–	0.0092	0.0025
Alanine	Poly-DL-alanine (in acetone)	–CH3	0.0074	0.0074
Isoleucine	Poly-L-isoleucine (in acetone)		0.016	0.016
Similar to:
Leucine
Valine
Proline
Lysine	Poly-l-lysine (in buffer)	–CH2–	0.0077	0.0073
Arginine	Poly-L-arginine (in DMFA)	–NH2	0.014	0.014
Histidine	Poly-l-histidine (in buffer)	–NH–	0.016	0.013
		=N–
		–CH=
Tryptophan	Poly-l-tryptophan (in acetone)	–NH–	0.0171	0.0087
		–CH=
Tyrosine	Poly-l-tyrosine (in buffer)	–OH	0.0022	0.0002
Threonine	Poly-L-threonine (in acetone)	–CH=	0.011	0.011
Glutamic acid	Poly-l-glutamic acid (in buffer)	–CH2–	0.0002	0.0002
		–COOH
Similar to:
Aspartic acid
Methionine	Poly-L-methionine (in acetone)	–CH2–	0.025	0.025
		–SH
Similar to: cystein