The Pepscan systems produced kinase array. Left.The design includes 1176 substrates that bar a number of spotting controls are derived from the PhosphoBase resource and thus represent peptides that in a bona fide kinase reaction are phosphorylated by a peptide. Right. The reaction involves the covalent transfer of the terminal phosphor group of ATP as arrays efficiently incorporated radioactivity if incubated with cell lysates and 33P-γ-ATP but failed to do so when incubated with cell lysates and 33P-α-ATP.
