Research Article
The Reporter System for GPCR Assay with the Fission Yeast Schizosaccharomyces pombe
Table 3
Primer information.
| | Primer name | Target gene | Regiona | Forward (5′3′) | Reverse (5′3′) |
| | pAL7inv | | | GAGCAAAAGGCCAGCAAAAG | AACCGTATTACCGCCTTTGA | | GFPORF | | | ATGGGCGTGATCAAGCCCG | TTAGCCGGCCTGGCGGGGT | | dhc1up | SPAC1093.06c/dhc1 | −1044 | AAGCACGCGCTCTAATTCAT | GGTGTCAAGAAAACTTGACCG | | dhc1dw | | +958 | AACTTGAAACTATTTGTTGTTTACTA | GAATCTGAGGTTGATGTTGAA | | mam2up | SPAC11H11.04/mam2 | −1068 | CATCGGGATTGCATTGAGAGT | AATGTCAGAGGGAGCAAGAACA | | mam2dw | | +1010 | CTTACGCCTGAATGTATCTTT | ACTCAAAGCCATAACTGTGC | | mam3up | SPAP11E10.02c/mam3 | −1041 | TTTTAGAAAGTGTCTATTGTACC | GACGAATTATGGGAAGATCAAG | | mam3dw | | +997 | ATAAAGTTAATGTTTTATATTTATTTTACA | ACTGAGAATGTCGTCTGTCC | | rgs1up | SPAC22F3.12c/rgs1 | −1118 | GGCAGGTGTAAGAAGCGTTG | CCAAAGCTGATTCTTACTTTTACGA | | rgs1dw | | +944 | TGCATAGAAAACAATCGTGT | CGAAAGAATCCTGCTGTTAC | | SPBC4.01up | SPBC4.01 | −985 | CCCATCTGGGTGAAAGAGTG | CCATTCTTAAACCGTAATTTTAAATTG | | SPBC4.01dw | | +992 | ACAAACATAAATAAGATTTTGTAAAC | AATTATTGCTGTCGCCGAAC | | spk1up | SPAC31G5.09c/spk1 | −1040 | GGACGCCAAGGGAAATTTAT | TAGACTACAAATTGAAAAACTTGAAAG | | spk1dw | | +958 | AAAGCTTCAACTAGAATTCTCCT | CAACCGATGACGGTATTTAT | | sxa2up | SPAC1296.03c/sxa2 | −1363 | AGATTATGGGGTAGTGGGTTC | GCATTGAAAAGAGAGACAATGA | | sxa2dw | | +945 | AAGTTTAATATCGGAAAATTTAA | CGGAAGTTAGGCTTGTGTGC |
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aRegion represents how distant from ATG or stop codon of its target gene is ORF.
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