SKIP Downregulation Increases TGF-β1-Induced Matrix Metalloproteinase-9 Production in Transformed Keratinocytes
Figure 5
The enhancement of ERK1,2-dependent signaling mediates the TGF-β1-increased MMP-9 expression in downregulated SKIP cells. (a) Inhibition of transcriptional activity of ERK1,2-dependent SRE-Luc reporter by PD98059 (25 M) in AS-S cells. (b) Gelatine-zymography assay and RT-PCR analysis in AS-S cotreated with the MEK1,2 inhibitor PD98059 and/or TGF-β1. Cells were treated for 48 h for zymography and 24 h for RT-PCR. GAPDH was amplified as RT-PCR control for the amount of cDNA present in each sample. (c) Gelatine-zymography assay of PDV/Mock control cells treated 48 h with TGF-β1 in the presence or absence of PD98059.
