Figure 6: Proteolytic activity of the extrinsic factor Xase formed by factor VIIa and relipidated placental TF ( glycosylated and deglycosylated), full-length recombinant TF 1–263 (■ glycosylated and □ deglycosylated) and truncated recombinant TF 1–243 ( native and ° “deglycosylated”). Factor VIIa (5 nM) was incubated with 0.1 nM relipidated TF and increasing concentrations of factor X were added. Factor Xa generation was monitored in a chromogenic assay. (This figure was originally published in J Biol Chem) [12].