Holocarboxylase Synthetase 1 Physically Interacts with Histone H3 in Arabidopsis
Figure 3
Avidin precipitation assays. The protein samples were analyzed by 15% SDS-PAGE. I125-streptavidin (left panel) and histone H3 antibody (right panel) were used in the western blots. Input: total protein extracted from Arabidopsis. B: blank control, where avidin beads were used only with release buffer. Sup: the supernatant after the avidin beads were incubated with Arabidopsis lysate overnight. W1/2/3: the supernatants for the first, second, and third washes separately after the incubation. AP: precipitation of total protein preformed with avidin beads. ACC1: homomeric acetyl-CoA carboxylase. MCC: methylcrotonyl-CoA carboxylase. BCCP1: biotin carboxyl carrier protein1, which is part of the heteromeric acetyl-CoA carboxylase. BCCP2: biotin carboxyl carrier protein2, which is part of the heteromeric acetyl-CoA carboxylase. Experiments were conducted in triplicate.
