Scientifica

Research Article

Radiosensitivity and Induction of Apoptosis by High LET Carbon Ion Beam and Low LET Gamma Radiation: A Comparative Study

Table 1

Cell cycle distribution in HeLa and HsiI cells by FACS. Cells were irradiated with different doses of carbon ion beam and gamma radiation (0–4 Gy). The percentages of cell cycle distribution were evaluated by ModFit LT software using PI staining. Values represent the mean ± SD of three independent experiments. values were calculated at 0.05 confidence level with respect to unirradiated control for each phase of each dose and shown within the brackets.

Dose
(Gy)

Irradiated with gamma

Irradiated with carbon

HeLa (% of cell population with standard deviation)

HsiI (% of cell population with standard deviation)

HeLa (% of cell population with standard deviation)

HsiI (% of cell population with standard deviation)

G₀/G₁

G₂/M

G₀/G₁

G₂/M

G₀/G₁

G₂/M

G₀/G₁

G₂/M

24.57 ± 0.33

59.77 ± 1.09

15.64 ± 0.80

19.46 ± 0.84

69.79 ± 1.89

10.74 ± 1.04

40.77 ± 0.36

43.87 ± 1.08

15.35 ± 0.72

37.33 ± 0.07

47.46 ± 0.38

15.19 ± 0.40

0.5

27.64 ± 0.17 (0.000)

57.35 ± 0.70 (0.114)

15.00 ± 0.62 (0.727)

21.05 ± 0.55 (0.041)

65.40 ± 1.48 (0.008)

13.54 ± 1.08 (0.013)

28.49 ± 0.16 (0.000)

53.58 ± 0.82 (0.013)

17.92 ± 0.78 (0.529)

34.30 ± 0.80 (0.009)

48.85 ± 1.58 (0.846)

16.84 ± 0.87 (0.510)

1.0

27.73 ± 0.55 (0.000)

56.65 ± 0.34 (0.038)

15.61 ± 0.40 (1.000)

26.54 ± 0.64 (0.000)

54.77 ± 0.90 (0.000)

18.68 ± 0.33 (0.000)

26.89 ± 0.27 (0.000)

57.57 ± 0.94 (0.001)

15.53 ± 1.11 (1.000)

30.69 ± 1.29 (0.000)

46.68 ± 1.27 (0.975)

22.62 ± 0.89 (0.000)

2.0

29.28 ± 0.65 (0.000)

53.72 ± 1.22 (0.000)

17.00 ± 0.57 (0.180)

24.12 ± 0.67 (0.000)

56.76 ± 1.40 (0.000)

19.11 ± 0.76 (0.000)

32.38 ± 1.22 (0.000)

55.87 ± 2.27 (0.003)

11.74 ± 1.11 (0.268)

19.16 ± 0.48 (0.000)

58.12 ± 0.71 (0.000)

22.71 ± 0.52 (0.000)

4.0

20.8 ± 0.90 (0.000)

59.66 ± 2.11 (1.000)

19.53 ± 1.27 (0.000)

20.16 ± 0.42 (0.509)

62.44 ± 0.68 (0.000)

17.39 ± 1.10 (0.000)

23.59 ± 1.50 (0.000)

47.39 ± 6.57 (0.501)

29.01 ± 5.08 (0.000)

34.16 ± 1.34 (0.007)

22.71 ± 4.32 (0.000)

43.12 ± 3.07 (0.000)