Gender Differences in Pulmonary Function, Respiratory Symptoms, and Macrophage Proteomics among HIV-Infected Smokers

Table 4

Proteins that had at least a 2-fold higher expression in alveolar macrophages from men.

Spot; number	Mean of normalized; volume of male	Mean of normalized; volume of female	Fold changes; (male/female)	Accession number; (Swiss-Prot)	Protein ID	Mowse; score	Observed pI/Mr; (KDr)	Number of peptides; by MS/MS	Total seq.; coverage (%)
867	0.04867	0.02083	2.34	NP_006079	Tubulin, beta, 2	2103	5.0/53.3	8	62
1009	0.05650	0.02167	2.61	BAD96752	Beta-actin variant	471	7.5/45.1	8	31
1318	0.12600	0.02983	4.22	P11177	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	427	6.2/39.6	4	39
1773	0.22083	0.06217	3.55	NP_001900	Cathepsin D preproprotein	898	5.2/27.7	5	33
1802	0.06200	0.03067	2.02	P30041	Peroxiredoxin 6	364	6.0/27.4	7	76
1872	0.05017	0.01600	3.14	P25787	Proteasome subunit alpha type 2	265	6.6/25.7	6	76
3074	0.17217	0.07350	2.34	1IVY_A	Chain A, Physiological Dimer Hpp Precursor	230	4.5/29.4	3	9
3157	0.06600	0.03250	2.03	P05092	Cyclophilin A	206	6.4/16.4	3	52
3235	0.11967	0.01050	11.40	P11021	78 kDa glucose-regulated protein	2193	5.3/74.9	24	50
4880	0.09550	0.00567	16.85	CAA68491	Glutathione peroxidase	1143	5.5/23.6	4	89
4925	0.12867	0.00100	128.67	P26447	Protein S100-A4	208	5.9/11.9	5	45
4990	0.11867	0.01933	6.14	P00558	Phosphoglycerate kinase 1	2011	7.9/44.9	13	72
5173	0.11133	0.03450	3.23	P30048	Thioredoxin-dependent peroxide reductase, mitochondrial	204	7.7/28.0	3	16
5180	0.10050	0.03300	3.05	Q06830	Peroxiredoxin-1	636	7.3/22.2	7	47
8849	0.06850	0.00100	68.50	P54727	UV excision repair protein RAD23 homolog B	643	4.8/43.2	8	41
8872	0.05933	0.02533	2.34	P06576	ATP synthase subunit beta, mitochondrial	513	5.3/56.5	8	34
8985	0.09233	0.01900	4.86	P06733	Enolase 1	638	5.7/42.3	8	28
9003	0.15800	0.03033	5.21	AAA51580	Gamma-actin	620	4.6/43.3	3	19
9023	0.63483	0.28000	2.27	Q3ZCM7	Tubulin beta-8 chain	344	4.8/49.7	4	18
9103	0.05133	0.01850	2.77	P06396	Gelsolin	1241	5.9/85.6	4	25
9126	0.06717	0.01100	6.11	O95336	6-phosphogluconolactonase	331	5.7/27.8	4	39
9141	0.10867	0.03400	3.20	1HJO_A	Chain A, Heat-Shock 70 kd Protein 42 kd Atpase N-Terminal Domain	1329	7.4/28.6	5	45
9152	0.08583	0.00217	39.62	P09668	Procathepsin H	517	8.4/37.4	3	29
9153	0.13433	0.01400	9.60	P52566	Rho GDP-dissociation inhibitor 2	616	5.1/23.0	10	75
9169	0.09733	0.04633	2.10	AAA35607	IgE-binding protein	257	7.4/26.1	2	22
9337	0.10317	0.04183	2.47	1GLO_A	Chain A, Crystal Structure Of Cys25ser Mutant Of Human Cathepsin S	193	6.6/16.5	5	32
9408	0.29833	0.12683	2.35	1AWI_A	Chain A, Human Platelet Profilin complexed with The L-Pro10 Peptide	988	9.1/14.5	2	93
9504	2.49950	1.10067	2.27	P08670	Vimentin	2541	5.1/53.6	12	63
9734	0.11817	0.00100	118.17	P10809	60 kDa heat shock protein, mitochondrial	2283	5.7/61.0	13	56

Only identified proteins are shown. Accession number: international database of the name and ID number of protein. Fold change: average density of the specific protein in males compared to females. Mowse score: measures reliability of the protein identification; greater than 100 ensures higher reliability that the protein identified is correct. Observed pI/Mr: isoelectric point and molecular weight of the protein. Ms/Ms: number of peptides sequenced after digestion. Total sequence coverage: percentage of the peptide sequenced.