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Scientifica
Volume 2014, Article ID 703136, 8 pages
http://dx.doi.org/10.1155/2014/703136
Research Article

Inducible Protective Processes in Animal Systems XIII: Comparative Analysis of Induction of Adaptive Response by EMS and MMS in Ehrlich Ascites Carcinoma Cells

Department of Studies in Bioscience, Post-Graduate Centre, University of Mysore, Hemagangotri, Hassan, Karnataka 573220, India

Received 25 January 2014; Revised 9 May 2014; Accepted 12 May 2014; Published 4 June 2014

Academic Editor: Nicolaas A. Franken

Copyright © 2014 Periyapatna Vishwaprakash Mahadimane and Venkateshaiah Vasudev. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In order to investigate the presence of adaptive response in cancerous cells, two monofunctional alkylating agents, namely, ethyl methanesulfonate (EMS) and methyl methanesulfonate (MMS), were employed to treat Ehrlich ascites carcinoma (EAC) cells in vivo. Conditioning dose of 80 mg/kg body weight of EMS or 50 mg/kg body weight of MMS and challenging dose of 240 mg/kg body weight of EMS or 150 mg/kg body weight of MMS were selected by pilot toxicity studies. Conditioned EAC cells when challenged after 8 h time lag resulted in significant reduction in chromosomal aberrations compared to challenging dose of respective agents. As has been proved in earlier studies with normal organisms, even in cancerous cells (EAC), there is presence of adaptive response to methylating and ethylating agents. Furthermore, it is also interesting to note in the present studies that the methylating agent, MMS, is a stronger inducer of the adaptive response than the ethylating agent, EMS.