Inducible Protective Processes in Animal Systems XIII: Comparative Analysis of Induction of Adaptive Response by EMS and MMS in Ehrlich Ascites Carcinoma Cells

Table 1

Frequency of chromosomal aberrations observed after conditioning, challenging, and combined treatments of EMS in EAC cells at 24 h recovery time.

Treatment	Chromosomal aberrations									Total; aberrations
	Exp. number	B′	B′′	RB′	RB′B′′	Minutes	Dic	Rings	ID
Control	1	8	—	—	—	1	—	—	—	9 (3.00)
	2	7	—	—	—	1	—	—	—	8 (2.67)
	3	6	—	—	—	—	—	—	—	6 (2.00)
	Mean ± SE	7.00±0.58	—	—	—	0.67±0.33	—	—	—	7.67 ± 0.88^a (2.56)
Conditioning	1	22	4	4	3	8	—	—	4	45 (15.00)
	2	23	3	3	3	6	1	—	2	41 (13.67)
	3	24	3	2	2	5	—	—	5	41 (13.67)
	Mean ± SE	23.00±0.58	3.33±0.33	3.00±0.58	2.67±0.33	6.33±0.88	0.33±0.33	—	3.67±0.88	42.33 ± 1.33^b (14.11)
Challenging	1	95	15	20	23	29	3	2	12	199 (66.33)
	2	86	13	21	21	20	2	1	8	172 (57.33)
	3	82	17	22	18	24	2	1	9	175 (58.33)
	Mean ± SE	87.67±3.84	15.00±1.15	21.00±0.58	20.67±1.45	24.33±2.60	2.33±0.33	1.33±0.33	9.67±1.20	182.00 ± 8.54^c (60.67)
Combined	1	65	11	19	20	20	2	1	8	146 (48.67)
	2	72	10	17	18	19	1	1	6	144 (48.00)
	3	68	10	20	15	16	2	1	5	137 (45.67)
	Mean ± SE	68.33±2.03	10.33±0.33	18.67±0.88	17.67±1.45	18.33±1.20	1.67±0.33	1.00±0.00	6.33±0.88	142.33 ± 2.73^d (47.44)

Note: data of 3 independent experiments; 3 animals per experiment were used; 100 cells per animal scored; and a total of 900 cells scored per dose. B′: chromatid break, B′′: isochromatid break, RB′′: chromatid exchange, RB′B′′: triradials, Dic: dicentrics, and ID: intrachromatid deletion. Values with same superscripts are not significantly different (𝑃>0.05), whereas values with different superscripts are significantly different (𝑃<0.05) from one another. Parentheses show the percentage of total aberrations.