Table 12: Advantages of XF H and E method.

Routine H and E1.5% DWS95% DLW

Cost High Low Low
Time 50–55 min 25–30 min 54 min
Toxicity Present Absent Absent
BiohazardousYes No No
Inflammability of chemicals used Present AbsentAbsent
Staining protocol Lengthy Simplified Simplified
Handling Toxic if not properly handledEasy Easy
Quality of stainingGoodGoodGood
Disposal of chemicals DifficultEasy Easy
PreparationSyntheticSyntheticNaturally available from plants