Effects of the Probiotic Enterococcus faecium and Pathogenic Escherichia coli Strains in a Pig and Human Epithelial Intestinal Cell Model

Table 1

Mannitol flux rates and their corresponding TEER values in IPEC-J2 cell monolayers incubated with various bacterial strains.

	Control	Ecf	ETEC	EPEC	Sampling time
TEER (Ohmcm^2)	4760 ± 1283	4744 ± 1187	3021 ± 2341	4590 ± 1263	4 h
Mannitol flux (nmolcm^−2h^−1)	4.4 ± 3.2	3.9 ± 3.7	6.5 ± 5.4	15.2 ± 13.8
TEER∣ Mannitol	146	157	149	126
TEER (Ohmcm^2)	3518 ± 1397^a	3692 ± 1177^a	2690 ± 2428^ab	1115 ± 1018^b	8 h
Mannitol flux (nmolcm^−2h^−1)	2.1 ± 2.6^a	3.4 ± 2.0^a	11 ± 12.2^ab	26.9 ± 25.1^b	8 h
TEER∣ Mannitol	138	147	134	116

Bacterial strains: IPEC-J2 cells were incubated with E. faecium (Ecf), ETEC, or EPEC for 4 h and 8 h (mean ± standard deviation, variance analysis, post hoc Scheffe test, , and different letters indicate significant differences between incubation groups); = cell culture inserts.