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Volume 2015, Article ID 802980, 7 pages
Research Article

Study of Collagen Birefringence in Different Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red and Polarized Light Microscopy

1Oral & Maxillofacial Pathology, Sri Sankara Dental College, Varkala, Akathumuri, Vennicode, Kerala 695318, India
2Oral & Maxillofacial Pathology, Sri Siddhartha Dental College & Hospital, Sri Siddhartha Academy of Higher Education, Tumkur 572107, India
3Oral & Maxillofacial Pathology, Vananchal Dental College & Hospital, Farathiya, Garhwa, Jharkhand 822114, India

Received 1 June 2015; Revised 28 September 2015; Accepted 30 September 2015

Academic Editor: Zhenhe Suo

Copyright © 2015 Pillai Arun Gopinathan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objectives. The present study was done to evaluate birefringence pattern of collagen fibres in different grades of oral squamous cell carcinoma using Picrosirius red stain and polarization microscopy and to determine if there is a change in collagen fibres between different grades of oral squamous cell carcinoma. Materials and Methods. Picrosirius red stained 5 μm thick sections of previously diagnosed different grades of squamous cell carcinoma and normal oral mucosa were studied under polarization microscopy for arrangement as well as birefringence of collagen fibres around tumour islands. Results. It was found that thin collagen fibres increased and thick collagen fibres decreased with dedifferentiation of OSCC (). It was observed that there was change in polarization colours of thick fibres from yellowish orange to greenish yellow with dedifferentiation of OSCC indicating loosely packed fibres (). Conclusion. There was a gradual change of birefringence of collagen from yellowish orange to greenish yellow from well to poorly differentiated squamous cell carcinoma, indicating that there is a change from mature form of collagen to immature form as tumour progresses. Studying collagen fibres with Picrosirius red for stromal changes around tumour islands along with routine staining may help in predicting the prognosis of tumour.