Simultaneous Quantification of Dexpanthenol and Resorcinol from Hair Care Formulation Using Liquid Chromatography: Method Development and Validation

Table 3

System suitability and method validation data for the simultaneous quantification of DP and RC.

Parameters	Resorcinol	Dexpanthenol
System suitability
Retention time (min)	7.19	3.19
Capacity factor	1.08	1.32
Peak resolution	15.41	—
Tailing factor	0.97	0.56
Theoretical plates ()
Sensitivity
LOD (µg·mL^−1)	2.19	0.62
LOQ (µg·mL^−1)	6.64	1.89
Precision
Intraday
Mean content (mg/100 mL) ()  ± % RSD	50.41 ± 0.19	49.77 ± 0.20
Interday
Mean content (mg/100 mL); (day 1/day 2/day 3) ()	50.40/50.38/50.39	49.76/49.78/49.65
(% RSD) (day 1/day 2/day 3) ()	0.20/0.19/0.21	0.11/0.19/0.15
Specificity
Peak purity angle	0.129	0.217
Peak purity threshold	0.337	0.411

With respect to DP.