Simultaneous Quantification of Dexpanthenol and Resorcinol from Hair Care Formulation Using Liquid Chromatography: Method Development and Validation

Table 4

Results of accuracy study.

Solution	% of nominal; value for RC	Concentration of final; solution in µg·mL^−1	Recovery; %	Observed concentration of; final solution (µg·mL^−1)	Average assay value; (mg·100 mL^−1)	Observed; recovery; %	Accuracy; %	% RSD
	100	40.00	—	40.33	50.42	—	—	0.20
RA	80	32.00	80.00	32.27	40.35	80.03	100.03
RB	110	44.03	110.07	44.93	56.17	111.40	101.21	0.82
RC	120	48.12	120.30	48.33	60.42	119.83	99.61
Solution	% of nominal; value for DP	Concentration of final; solution in µg·mL^−1	Recovery; %	Observed concentration of; final solution (µg·mL^−1)	Average assay value; (mg·100 mL^−1)	Observed; recovery; %	Accuracy; %	% RSD
	100	40.00	—	39.82	49.77	—	—	0.02
DA	80	32.00	80.00	31.88	39.84	79.91	99.89
DB	110	44.12	110.30	44.05	55.09	110.69	100.35	0.48
DC	120	47.99	119.97	47.69	59.61	119.77	99.83

Note: average from three replicate injections from sample preparations.
The recovery study is carried out over three-concentration range.