Research Article
A FACS Based Case Study on Two HbE-β Thalassaemia Members of a Family, Having Similar Mutational Background
Table 3
Optimized component concentrations for PCR.
| Components | Concentration | Volume/reaction |
| PCR buffer with MgCl2 | 10x | 2.5 L | dNTPs | 2 mM | 2.5 L | Forward primer | 10 pm/L | 1 L | Reverse primer | 10 pm/L | 1 L | Taq polymerase | 3 U/L | 0.4 L | PCR water | | 15.6 L | Genomic DNA | 50 ng/L | 2 L | Total | | 25 L |
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