Research Article
A FACS Based Case Study on Two HbE-β Thalassaemia Members of a Family, Having Similar Mutational Background
Table 4
Cycling conditions for PCR.
| | Condition | Temperature | Time | Number of cycles |
| | Initial denaturation | 96°C | 5 minutes | — |
| | Denaturation | 94°C | 30 seconds | 30 | | Annealing | 50°C | 30 seconds | | Extension | 72°C | 30 seconds |
| | Final extension | 72°C | 10 minutes | — |
| | Final hold | 4°C | | — |
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