Research Article
KCl-Dependent Release of Mitochondrial Membrane-Bound Arginase Appears to Be a Novel Variant of Arginase-II
Figure 1
Flow chart of the procedure of isolation of cytosolic fraction, mitochondrial fraction, and mitochondrial outer membrane-bound fraction. The experimental set 1 mitochondria were isolated with KCl in homogenizing medium and experiment set 2 in which mitochondria were isolated first and then washed with different concentration of KCl in homogenizing buffer.