A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice

Figure 2

Effects of a high-fat, high-fructose diet on CuZn-SOD, Mn-SOD, CAT, and GPx mRNA expression in mouse livers. Mice were fed a commercial standard diet for 4 weeks, and a high-fat, high-fructose diet (HFFD) was administered intragastrically for 2, 4, or 8 weeks. A semiquantitative determination of the hepatic mRNA expression of certain genes was performed using pairs of primers specific to the investigated genes. The data are presented as the mean ± SD (). Significant differences were determined using a one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. versus the regular diet mice; versus 2-week HFFD mice; versus 4-week HFFD mice.