A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice

Figure 4

Effects of a high-fat, high-fructose diet on the total GSH, reduced GSH, and GSSH content and the GSH/GSSG ratio in the mouse livers. Mice were fed a commercial standard diet for 4 weeks, and a high-fat, high-fructose diet (HFFD) was administered intragastrically for 2, 4, or 8 weeks. The mice were sacrificed 24 h after the last treatment, and their livers were immediately excised to examine the total glutathione, reduced GSH (black column), and GSSG (white column) contents, and the GSH/GSSH ratio was calculated (digit above the graph). The data are presented as the mean ± SD (). Significant differences were determined using a one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. versus the regular diet mice.