Research Article
A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice
Table 2
PCR programs for mouse CAT, CuZn-SOD, Mn-SOD, GPx, and GAPDH.
| Gene | Denaturation (°C/sec) | Annealing (°C/sec) | Extension (°C/sec) | Number of cycles | Product size (bp) | References |
| CAT | 94/45 | 55/60 | 72/60 | 31 | 229 | [17] | CuZn-SOD | 94/45 | 56/60 | 72/60 | 26 | 246 | [18] | Mn-SOD | 94/45 | 55/60 | 72/60 | 35 | 241 | [18] | GPx | 94/45 | 56/60 | 72/60 | 28 | 197 | [18] | GAPDH | 95/30 | 64/30 | 72/60 | 28 | 145 | [19] |
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