Research Article
Effects of Cadmium and Zinc on the Gamete Viability, Fertilization, and Embryonic Development of Tripneustes gratilla (Linnaeus)
Figure 1
Results of RRT performed on T. gratilla sperm cells treated with (a) CdCl2 and (b) ZnSO4, and egg cells (c and d) treated with the same heavy metal solutions, respectively. M0 (media control), PC (positive control), T1 (1 M), T2 (0.5 M), T3 (0.1 M), T4(5 × 10−2 M), T5 (1 × 10−2 M), T6 (5 × 10−3 M), T7 (1 × 10−3 M), T8 (5 × 10−4 M), T9 (1 × 10−4 M), and NC (negative control). R1–R3 are the replicate wells. (e) Mean absorbance at 630 nm of the wells containing T. gratilla gametes with CdCl2 and ZnSO4 treatments after 30 mins of incubation with resazurin. (f) A histogram showing the mean absorbance at 630 nm of the wells containing T. gratilla gametes with CdCl2 and ZnSO4 treatments after 30 mins of incubation with resazurin.
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