Antinociceptive Effect of Tephrosia sinapou Extract in the Acetic Acid, Phenyl-p-benzoquinone, Formalin, and Complete Freund’s Adjuvant Models of Overt Pain-Like Behavior in Mice

Figure 1

T. sinapou ethyl acetate extract inhibited writhing response in mice. Panel (a): mice were treated i.p. with T. sinapou ethyl acetate extract (1–100 mg/kg, 30 min), indomethacin (indo, 5 mg/kg, i.p. 40 min), or vehicle before i.p. stimulus with acetic acid (0.8% diluted in saline). Panel (b): mice were treated with 100 mg/kg of T. sinapou ethyl acetate extract (30 min) through i.p., p.o., and s.c. routes and indomethacin (indo, 5 mg/kg, i.p. 40 min) or vehicle. Panel (c): mice were treated i.p. with T. sinapou ethyl acetate extract (1–100 mg/kg, 30 min), indomethacin (indo, 5 mg/kg, i.p. 40 min), or vehicle before phenyl-p-benzoquinone (PBQ, 1890 μg/kg diluted in 2% DMSO in saline) stimulus. Writhing score was evaluated during 20 min after stimulus injection. Experiments were performed with 6 mice per group per experiment and are representative of 2 independent experiments. compared to the saline group, compared to the vehicle group, compared to the vehicle group, the dose of 1 mg/kg (panels (a) and (c)), 3 mg/kg (panel (c)) of the extract, and s.c. route (panel (b)), and compared to the vehicle group and the doses of 1–10 mg/kg of extract (panel (a)), the doses 1–30 mg/kg (panel (c)), and s.c. and p.o. routes of administration.

(a)

(b)

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